Journal: Molecular Medicine

Article Title: Chemerin alleviates the placental oxidative stress and improves fetal overgrowth of gestational diabetes mellitus mice induced by high fat diet

doi: 10.1186/s10020-024-01007-2

Figure Lengend Snippet: The effects of different concentrations of chemerin on ROS level, T-AOC and glucose uptake in the high glucose cell model. ( A ) The relative fluorescence intensity of ROS. ( B ) The detection of total antioxidant capacity. ( C ) The intake of glucose by cells. HG: high glucose; ROS: reactive oxygen species; T-AOC: total antioxidant capacity. Data were shown as mean ± SD ( n ≥ 3). * P < 0.05; ** P < 0.01

Article Snippet: For treatment, pregnant mice were intraperitoneally injected the active recombinant human chemerin (10 μg/kg, HY-P70099, MedChemExpress, USA) dissolved in 200 μL saline every day during D12.5∼D15.5, while mice of the other group was injected intraperitoneally with 200 μL saline as control.

Techniques: Fluorescence

Journal: Molecular Medicine

Article Title: Chemerin alleviates the placental oxidative stress and improves fetal overgrowth of gestational diabetes mellitus mice induced by high fat diet

doi: 10.1186/s10020-024-01007-2

Figure Lengend Snippet: The effects of chemerin on the glucose tolerance, weight of fetal mice and placentas, fasting insulin levels, lipid metabolism and liver function. ( A ) The blood glucose of pregnant mice detected by IPGTT on D16.5 (* represents the comparison between the chow groups and the HFD groups; # represents the comparison between the HFD groups and the treated groups). ( B ) The body weight of fetal mice on D18.5. ( C ) The weight of mice placentas on D18.5. ( D ) The sizes of fetal mice and placentas on D18.5 after chemerin treatment. ( E ) The serum level of chemerin on D18.5. ( F ) The serum level of fasting insulin on D18.5. ( G ) The serum level of ALT on D18.5. ( H ) The serum level of AST on D18.5. ( I ) The serum level of HDL on D18.5. ( J ) The serum level of LDL on D18.5. ( K ) The serum level of TC on D18.5. ( L ) The serum level of TG on D18.5. HFD: high fat diet; FINS: fasting insulin; ALT: alanine aminotransferase; AST: aspartate transaminase; HDL: high-density lipoprotein; LDL: low-density lipoprotein; TC: total cholesterol; TG: triglyceride. Data were shown as mean ± SD ( n ≥ 3). # P < 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: For treatment, pregnant mice were intraperitoneally injected the active recombinant human chemerin (10 μg/kg, HY-P70099, MedChemExpress, USA) dissolved in 200 μL saline every day during D12.5∼D15.5, while mice of the other group was injected intraperitoneally with 200 μL saline as control.

Techniques: Comparison

Journal: Molecular Medicine

Article Title: Chemerin alleviates the placental oxidative stress and improves fetal overgrowth of gestational diabetes mellitus mice induced by high fat diet

doi: 10.1186/s10020-024-01007-2

Figure Lengend Snippet: The localization and expressions of chemerin and DsbA-L in mice placenta of the HFD groups and the chow groups. ( A ) Staining of chemerin and DsbA-L by immunohistochemistry (scale bar = 100 μm). ( B ) Western blotting images of mice placentas. ( C ) Protein quantification of chemerin relative to β-actin. ( D ) AOD of chemerin in mice placenta. ( E ) AOD of DsbA-L in mice placenta. DsbA-L: disulfide-bond A oxidoreductase-like protein; HFD: high fat diet; LA: labyrinth; JZ: junctional zone; AOD: average optical density. Data were shown as mean ± SD ( n ≥ 3). ** P < 0.01, **** P < 0.0001

Article Snippet: For treatment, pregnant mice were intraperitoneally injected the active recombinant human chemerin (10 μg/kg, HY-P70099, MedChemExpress, USA) dissolved in 200 μL saline every day during D12.5∼D15.5, while mice of the other group was injected intraperitoneally with 200 μL saline as control.

Techniques: Staining, Immunohistochemistry, Western Blot

Journal: Molecular Medicine

Article Title: Chemerin alleviates the placental oxidative stress and improves fetal overgrowth of gestational diabetes mellitus mice induced by high fat diet

doi: 10.1186/s10020-024-01007-2

Figure Lengend Snippet: The roles and mechanisms of chemerin in the placental oxidative stress and insulin resistance in HFD pregnant mice. ( A ) Western blotting images of mice placentas of three groups. ( B ) Western blotting images of mice placentas between the chow groups and the HFD groups. ( C ) Western blotting images of mice placentas between the HFD groups and the treated groups. The HFD placenta samples presented in ( B ) and ( C ) were from the same several mice. ( D ) Protein quantification of AKT relative to β-actin. ( E ) Protein quantification of IRS1 relative to β-actin. ( F ) Protein quantification of STING relative to β-actin. ( G ) Protein quantification of DsbA-L relative to β-actin. ( H ) The activity of SOD in mice placenta. ( I ) The activity of GSH-Px in mice placenta. ( J ) The activity of CAT in mice placenta. ( K ) The content of MDA in mice placenta. HFD: high fat diet; AKT/PKB: protein kinase B; IRS1: insulin receptor substrate 1; STING: stimulator of interferon genes; DsbA-L: disulfide-bond A oxidoreductase-like protein; SOD: superoxide dismutase; GSH-Px: glutathione peroxidase; CAT: catalase; MDA: malondialdehyde. Data were shown as mean ± SD ( n ≥ 3). * P < 0.05; ** P < 0.01

Article Snippet: For treatment, pregnant mice were intraperitoneally injected the active recombinant human chemerin (10 μg/kg, HY-P70099, MedChemExpress, USA) dissolved in 200 μL saline every day during D12.5∼D15.5, while mice of the other group was injected intraperitoneally with 200 μL saline as control.

Techniques: Western Blot, Activity Assay

Journal: Molecular Medicine

Article Title: Chemerin alleviates the placental oxidative stress and improves fetal overgrowth of gestational diabetes mellitus mice induced by high fat diet

doi: 10.1186/s10020-024-01007-2

Figure Lengend Snippet: The long-term impacts of chemerin on blood glucose levels and body weight of maternal and fetal mice. ( A ) The postpartum blood glucose levels of the maternal mice at 8 weeks by IPGTT. ( B ) The blood glucose levels of the mice offspring at 8 weeks old by IPGTT. ( C ) The serum levels of chemerin in the maternal mice at 8 weeks after delivery. ( D ) The serum levels of chemerin in the mice offspring at 8 weeks old. ( E ) The changes of body weight of the mice offspring from 1 to 8 weeks of age. HFD: high fat diet. Data were shown as mean ± SD ( n ≥ 3). * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: For treatment, pregnant mice were intraperitoneally injected the active recombinant human chemerin (10 μg/kg, HY-P70099, MedChemExpress, USA) dissolved in 200 μL saline every day during D12.5∼D15.5, while mice of the other group was injected intraperitoneally with 200 μL saline as control.

Techniques: